trailblazers michael beasley jermaine jones hbo luck dwight howard unc asheville stephen jackson
Wednesday, May 2, 2012
Grandparents Give Greatest Wedding Toast Ever
Old people. They're the best.
Mike is about to marry Sue, and in this video, his grandparents offer perhaps the greatest wedding toast of all time. Among their pieces of advice: Be good to each other, help each other financially, and make sure you have food in your Frigidaire.
Also, be sure to travel together a lot, but watch out for those topless European girls, and don't argue with each other ... except when you're arguing about arguing.
A little PDA never hurts either, even after 72 years. To the happy couple!
titanic ii babe ruth new jersey nets nba playoff schedule rondo morris claiborne mothers day gifts
The TechCrunch Meet-Up In NYC Is Officially Official: RSVP Now
As the days pass, the big moment draws nigh. No, I'm not talking about Disrupt, which is a big moment in its own right, nor am I teasing any other big launch. I'm talking about a party. A huge party. We initially called it a mini meet-up ? a gathering of New York's tech startup scene, including investors, entrepreneurs and TechCrunch editorial ? but its grown into so much more than that. With over 600 RSVPs, ten sponsors, a badass venue, and more TC staff in attendance than we'd originally expected, our mini meet-up is now massive.old school nick swisher jaco san jose sharks humber perfect game ufc 145 fight card ufc145
Tuesday, May 1, 2012
Unknown function of transcriptional regulator of development and cancer reveaked: Jarid2 may break the polycomb silence
ScienceDaily (Apr. 30, 2012) ? Historically, fly and human Polycomb proteins were considered textbook exemplars of transcriptional repressors, or proteins that silence the process by which DNA gives rise to new proteins. Now, work by a team of researchers at the Stowers Institute for Medical Research challenges that dogma.
In a cover story in the May 2012 issue of the journal Molecular and Cellular Biology, Stowers Investigator Ali Shilatifard, Ph.D., and his team report that in the fruit fly Drosophila melanogaster a component of the Polycomb repressive complex 2 (PRC2), which is called Jarid2, occasionally activates gene expression.
Polycomb proteins, a group of chromatin-binding factors, were initially found to control how developing fly embryos undergo segmentation. Subsequently, researchers discovered that humans also express a gaggle of proteins resembling fly Polycomb factors and that these proteins have crucially significant roles: under normal conditions PRC2 proteins are required for the differentiation of stem cells, but when overactive, act as oncogenes in lymphoma, breast, and prostate cancer.
"Our approach is to use Drosophila as a model to learn how Polycomb group proteins function in the context of cancer," says Shilatifard, who studies mechanisms underlying leukemia in yeast, fruit flies, and mammalian cells. "In this study, we found that a new component of the PRC2 complex, Jarid2, maintains repression of some genes, but also potentially activates other target genes."
In the usual fly-to-human discovery continuum, the current study represents a species turnaround. While investigators analyzing mammalian stem cells had shown that Jarid2 was an important component of PRC2 in mammals, its activity remained unknown in Drosophila. To determine whether it acted together with Polycomb PRC2 in flies, the Stowers team, led by postdoctoral researcher Hans-Martin Herz, Ph.D., applied a proteomic approach to enumerate every Drosophila protein binding to Jarid2 in fly embryos.
Predictably, they found that fly Jarid2 associates with the same protein suspects as it does in mammals. "Previous mammalian studies reported opposing findings in regard to the role of Jarid2 in the PRC2 complex," says Herz. "Some papers said Jarid2 inhibited PRC2 activity while others claimed Jarid2 activated it."
To settle the issue, the group undertook genome-wide profiling by scanning the entire fruit fly genome to determine where Jarid2 and other members of the PRC2 complex localized on DNA. Overall, the proteins showed similar distribution patterns along chromosomes, hinting that PRC2 complexes containing Jarid2 regulate many of the same genes. However, some genes known to be PRC2 targets showed very little occupancy by Jarid2, an observation that suggested Jarid2 does not share all of the characteristics of a core member of the PRC2 complex.
Combining state-of-the-art genomic approaches with classic fly genetics, the group then defined what Jarid2 actually does molecularly in cells. To do that, they compared gene expression profiles of larvae of two distinct types of genetically manipulated flies: those that lacked only Jarid2 expression and flies that lacked expression of a core PRC2 protein. The rationale was that if Jarid2 and the complex shared the same targets and acted in lockstep, those profiles should be identical.
Two surprising observations emerged from that study. First, in Jarid2 mutant flies, some Jarid2/PRC2 co-bound genes exceeded their normal expression and others were inhibited, the latter suggesting that Jarid2 does not always repress a target. Secondly, the pattern of gene expression observed in the Jarid2 flies differed significantly from that seen in the PRC2 mutants, meaning that whatever Jarid2 does biochemically does not mimic what other PRC2 proteins do.
"Our findings challenge the general idea that PRC2 has only a repressive role," says Herz. "In fact, they suggest that PRC2 and Jarid2 may regulate different genes in specific contexts."
Recent reports implicate Jarid2 in diseases as diverse as multiple sclerosis and schizophrenia. But most significantly for the Shilatifard lab are studies linking both PRC2 and Jarid2 to myeloid cancers such as leukemia. "The theme of our lab is factors that regulate the yin and yang of transcription," says Shilatifard, citing his interest in both of the Polycomb proteins and their polar opposites, the Trithorax activator proteins -- initially characterized in flies and also linked to leukemia. "Our overall goal is to gain insight into both."
Also contributing to the study were Man Mohan, Ph.D., Alexander S. Garrett, Caitlynn Miller, David Casto, Ying Zhang, Christopher Seidel, Ph.D., Jeffrey S. Haug, Ph.D., Laurence Florens, Ph.D., Michael P. Washburn, Ph.D., of the Stowers Institute for Medical Research, as well as Masamitsu Yamaguchi, Ph.D., from Kyoto Institute of Technology, and Ramin Shiekhattar, Ph.D., from the Wistar Institute.
Funding for the study came from the Stowers Institute for Medical Research, the National Institutes of Health and The Jane Coffin Childs Memorial Fund for Medical Research.
Share this story on Facebook, Twitter, and Google:
Other social bookmarking and sharing tools:
Story Source:
The above story is reprinted from materials provided by Stowers Institute for Medical Research, via Newswise.
Note: Materials may be edited for content and length. For further information, please contact the source cited above.
Journal Reference:
- Zhuojuan Luo, Chengqi Lin, Erin Guest, Alexander S. Garrett, Nima Mohaghegh, Selene Swanson, Stacy Marshall, Laurence Florens, Michael P. Washburn, and Ali Shilatifard. The SEC family of RNA Polymerase II elongation factors: gene target specificity and transcriptional output. Mol. Cell. Biol., 30 April 2012 DOI: 10.1128/MCB.00182-12
Note: If no author is given, the source is cited instead.
Disclaimer: This article is not intended to provide medical advice, diagnosis or treatment. Views expressed here do not necessarily reflect those of ScienceDaily or its staff.
f 18 jet crash in virginia beach john tortorella nicki minaj beez in the trap video food network good friday f/a 18
Childish Gambino Dodges Ninjas While Talking Ninja Turtles
On our First Date, we learn the five-year plan of the actor/rapper also known as Donald Glover.
By Rya Backer
Childish Gambino
Photo: MTV News
I never thought I'd seek refuge in Childish Gambino.
OK, let me explain. After years of pursuing the multitalented, infinitely hilarious Donald Glover for a First Date, our stars crossed at long last, and we were able to get to know each other at Tribeca's Ninja restaurant. There, serving up our Japanese food, were men in full ninja regalia, terrifying us at every turn. Whether it was brandishing a dagger as we headed to our table, jumping in front of elevator doors as they opened, throwing down a ninja star on the table before making a dessert or slinging a yellow water balloon (yes, you read right) at us mid-meal, it made for a dining experience that required a companion willing to commiserate in all the scare tactics we came upon.
It was a fitting place to go, considering Glover is at the center of a grass-roots fan campaign to star as Donatello in the live-action remake of the "Teenage Mutant Ninja Turtles" movie (no doubt inspired by his own crusade to nab the title role in "The Amazing Spider-Man"). Midway into the meal, he resolved that he'd make a good Donatello, as he tongue-in-cheekily bragged about his prowess in instilling fear in others. But a role as a hero in a half-shell isn't the only aspiration on his horizon.
When asked where he sees himself in five years, he said, "I hope just successful. Still doing stuff I want to do. I always wanted to just do whatever I wanted whenever I wanted. I never really expected rap to be anything, I don't know if I'll still be rapping, but it'd be nice to still be making music on some level."
We can't wait to see — and hear — how he'll get there.
Do you want to see Donald Glover as a Ninja Turtle? Let us know in the comments!
Related Videos Related Artistslytro camera andrew brietbart branson mo monkees songs danica patrick school closings top chef texas
Subscribe to:
Posts (Atom)